Key elements to avoid contamination at the Molecular Biology Laboratory in Holguin province

Abstract

Introduction: The polymerase chain reaction (PCR) technique is a highly specific but very sensitive diagnostic tool. Its susceptibility to contamination is the main problem in laboratories. Therefore, taking into account the possible causes of its origin, preventing it and having several methods for its elimination is of paramount importance.

Objective: To describe the key elements to avoid contamination at the Molecular Biology Laboratory in Holguin province.

Methods: A literature review was conducted in the Google Scholar database and in relevant articles related to the topic. In addition, the Laboratory biosafety guidance related to coronavirus disease (COVID-19) version 2021 was used as a reference.

Information, analysis and synthesis: It is recommended to conduct an institutional risk assessment to identify the specific hazards of each stage of the process: exposure to aerosols, possible splashes, material spills and chemical hazards related to reagents. Preventing contamination of a PCR starts with the layout of the areas, that is why the workflow in a molecular biology laboratory is only in one direction: from pre-PCR to PCR. In addition to the application of general standards, other hazard elimination measures such as engineering control, germicidal efficacy of ultraviolet light, and laboratory contamination control must be taken.

Conclusions: Avoiding contamination in the Molecular Biology Laboratory in Holguin province in the course of the SARS-CoV-2 pandemic is vital for the quality of health services.