Bacterioma in Mammary Nodules and Their Morphological Effects on Breast Cancer Cell Line

Abstract

Introduction: No bacterium has been reported as causative of breast cancer, but the presence of different species that are related in a beneficial or detrimental way.

Objective: To identify the microbiota present in breast nodules and its morphological effects on the MCF-7 cell line.

Methods: Exploratory, experimental study of a population of 57 women with breast nodules, operated for needle biopsy in a diagnostic center. Of these, 17 samples were chosen by non-probabilistic sampling for metagenomic analysis. Infection was performed with MCF-7 cells and Staphylococcus saprophyticus at MOI of 1:1, 5:1 and 10:1 (48 hours of exposure).

Results: Of the 57 samples taken, only 7 yielded positive results (12.28%) and the rest (50) had no bacterial growth. The following microbiota was obtained by metagenomics: Proteobacteria (47%), Escherichia (9.4%) and Yokenella (8.2%), among others. Controls were negative. Only two patients tested positive for cancer, and S. saprophyticus was the common species. In the infection, morphological changes were evidenced from MOI 5:1.

Conclusion: The bacteriome extracted from the nodules of a female population is mostly endogenous flora of the mammary glands.