Advances in the microbiological diagnosis of human brucellosis in Cuba (2012-2021)
Keywords:
brucellosis, serological diagnosis, molecular diagnosis.Abstract
Introduction: Microbiology diagnosis of human brucellosis disappeared in Cuba in the 1990s. To reinstate it and confirm the cases, the National Reference Laboratory proposed to evaluate and implement new serological and molecular techniques to strengthen the working algorithm.
Methods: Seven studies (2012-2020) were performed in services with serological (FAB, HTFA, FAB microtechnique, Brucellacapt®) and molecular (bcsp PCR, Brucella Real-TM PCR, and Bruceladder mPCR) systems that allowed direct and indirect detection of Brucella spp.
Results: The performance of the serological methods evaluated exceeded 90%. PCR-BCSP excelled in the confirmation of serologically negative cases (85%), while the Brucella Real-TM system confirmed 60% of PCR-BCSP negative sera. In addition, molecular evidence was obtained for the presence of Brucella canis and Brucella abortus (vaccine and field strains) in Cuban human and animal clinical samples.
Conclusion: The described diagnostic methodologies constitute the first serological-molecular algorithm for the screening and diagnosis of human brucellosis in Cuba. This is a tool that provides the essential information for the adoption of strategies aimed at preventing this zoonosis in our country.
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