Procedure optimization for concentration and detection of protozoa and helminths in great volumes of water

Sandra Ríos Tobón, Juliana López Jiménez, Laura Francisca Campo Polanco, Ruth Marina Agudelo Cadavid, Lina Andrea Gutiérrez Builes

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Resumen

Feces-contaminated water is a vehicle of transmission of potentially pathogenic microorganisms responsible for illnesses that represent main causes of death worldwide. A protocol for detection of intestinal parasites in great volumes of water was optimized. It includes: membrane filtration, mechanical agitation with detergent, centrifugation, chemical concentration with Mini Parasep® and microscopic examination. From samples of feces-contaminated water containing parasitic forms, a total recovery percentage of 85.7 % of parasites was achieved after tests. This procedure provides a useful alternative method that could be subjected to validation as a routine methodology in the diagnosis of microbiological water quality.

Palabras clave

parasitology; waterborne diseases; water quality

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Copyright (c) 2018 Sandra Ríos Tobón, Juliana López Jiménez, Laura Francisca Campo Polanco, Ruth Marina Agudelo Cadavid, Lina Andrea Gutiérrez Builes

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Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.